UK DRI is seeking a Research Fellow to join Giles Hardingham's Lab at the Centre for Discovery Brain Sciences at the University of Edinburgh. You will drive a project aiming to determine how astrocytes change in response to Aß and tau pathology, the role that microglia play in mediating these changes, and the consequences of these changes. Ultimately this will lead to rescue strategies and therapeutic targets to modify patho-progression. The successful candidate will use established in vivo models of ß-amyloidopathy and tauopathy, including genetic models as well as the administration of human brain AD homogenate, plus mice lacking microglia (with and without microglial replacement therapy). Approaches will be tailored to the questions being asked, but may involve single-cell and spatial transcriptomics/proteomics, in vivo optical imaging, PET/MR, behavioural testing, electrophysiology and cell biological/primary culture approaches. Ref: 756, Closing date: 24 July 2023
Apply to centreUK DRI is seeking an experienced Senior Research Technician to provide technical support and management on projects related to the human neuropathology of Alzheimer’s disease in Prof Paul Matthews’ group at Imperial College London. This project intends to apply multi-omic cellular neuropathology methods to study brain tissues carrying a range of defined genetic risk variants to understand the earliest mechanisms of Alzheimer’s disease. The post holder will gain or extend experience by performing a range of cellular and/or molecular methods for brain tissue analysis, including immunohistochemistry, immunofluorescence, CLARITY or tissue expansion methods, RNAScope and related in situ hybridisation methods, next generation RNA sequencing, single cell or single nuclear RNA sequencing and ATAC sequencing. We now routinely integrate imaging mass cytometry methods for highly multiplexed cellular marker characterisation and will continue to expand this advanced capability. You will hold a degree level qualification in a science subject, experience with histology methods, collection, preparation, processing and/or storage of archived tissue specimens, with genomics methods and biological tissue management and methodical approach to work, good time management and record keeping. Ref: MED03868, Closing date: 06 June 2023
Apply to centreUK DRI is seeking a Research Fellow to join the Bartels Lab at UCL School of Life & Medical Sciences. One research project will involve the role of lipids in the physiological/pathological assembly of α-Synucleinin neuronal cells and the transition of liquid-liquid phase separation in neurodegeneration and synaptic transmission. You will be involved in analysis of protein mobility and assembly via live-cell microscopy and FRAP/FRET. The second project involves investigating the gut-brain axis in PD using mouse models and human synucleinopathy tissue; you will need to be familiar with at least one of the following techniques: protein biochemistry (Western, ELISA, IHC), biophysical structure work, protein purification, CryoEM, RT-QuiC/SAA. You will have a PhD or MD in Biophysics, Biochemistry or Molecular Biology, be a good communicator, have strong interpersonal skills, and be a proactive and creative thinker. Experience of working in quantitative biological analytics, or with biophysical structure analysis of proteins, handling human samples, or with FRET/FRAP is highly desirable. Ref: B02-04149, Closing date: 18 June 2023
Apply to centreUK DRI is seeking a Research Fellow in Cognitive Neuroscience to join the Wiseman Lab at School of Life & Medical Sciences at UCL. You will oversee and develop a novel and robust pipeline for the quantification of mouse behaviour and cognition for the assessment of mouse models of dementia-causing diseases, at the MRC Harwell Mary Lyon Centre (MLC). You will work with Dr Wiseman and the wider Institute to integrate this data with other research outputs at the UK DRI to provide ground-breaking insight into neurodegenerative mechanisms. You will also have the opportunity to develop new analytical tools to identify and classify changes in rodent behaviour, using DeepLabCut and AI clustering methods in collaboration with UK DRI colleagues. Work will focus on novel knockin mouse models of aspects of frontotemporal dementia and Alzheimer’s disease. You will have a PhD in Neuroscience and experience of the experimental design of mouse studies and the delivery of such studies under UK law. Experience in statistical analysis of experimental datasets using R or SPSS is essential, as is an understanding of and interest in the mission of the UK DRI and excellent communication, interpersonal, and time management skills. Closing date: 18 June 2023, Ref: B02-04988
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