Intracerebral haemorrhage in the striatum model

Stuart Allan

University of Manchester

Josie Thomas

josephine.thomas@manchester.ac.uk

Supplier

Charles River

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Haemorrhagic

https://drive.google.com/open?id=1JR04N9Fcdl26K7-vp1pTxhdP-syx3X5J

8-10 weeks

Isoflurane. 5% to induce, maintained at 2-2.5% throughout surgery

Topical EMLA. 0.06mg/kg buprenorphine administered s.c. Immediately following anaesthetic induction

None

Male

8-10 weeks

Up to 14 days

Standard chow - for 72h post-surgery animals receive normal diet pellets softened with water to improve feeding

Group housing, 2-5 animals/cage. Enrichment with sizzle nesting, chewing sticks, play tunnels, and seeds

Weight, modified neurological severity score, Behaviour (cylinder test, corner turn test), tissue pathology using immunohistochemistry and MRI (T2, dynamic contrast enhanced to assess BBB integrity, neurite orientation dispersion and density imaging to assess diffusion metrics and tissue microarchitecture), brain and systemic inflammation (cytokine assays).

https://drive.google.com/open?id=1JR04N9Fcdl26K7-vp1pTxhdP-syx3X5J

- Appropriate modelling of haematoma expansion (unlike autologous blood model)
- Straightforward protocol and relatively easy surgery to perform
- Could be easily modified to target other brain regions e.g. cortex, internal capsule, cerebellum
- Induces behavioural deficits lasting at least 14 days
- Largely consistent bleed sizes

- Collagenase induces rupture of multiple small blood vessels at injection site rather than a single vessel (artery) as seen in human ICH
- Possible concerns of inflammatory actions of collagenase which may exacerbate endogenous inflammatory mechanisms