- Overview
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Species
MouseStrain common name
-Full nomenclature
-Genetic background
C57BL/6JOriginal publication of model
DOI: 10.1242/dmm.029108Type of model
Ischemic stroke - Availability
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Principal investigator
Claire GibsonInstitution
University of NottinghamNamed contact
Claire GibsonContact email
claire.gibson@nottingham.ac.ukSource of model
SupplierSupplier code
Charles RiverMTA Holder and Issuing Organisation:
- - Intervention details
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Intervention
OcclusionStandard Operating Procedures for Intervention
-Age at Surgery
12 weeksAnaesthetic
IsofluraneAnalgesics
Carprieve (10 mg/kg, 5% w/v, Norbrook Laboratories Ltd., UK) injected subcutaneously immediately prior to undergoing MCAO surgery along with Marcaine (2 mg/kg, 0.25% w/v, AstraZeneca, UK) applied directly to the skin surrounding the wound site at the poinAntibiotics
NoneDuration of occlusion is temporary
Method for inducing the occlusion is mechanical
Mechanical Occlusion Type is filament (doccol filament)
- Study details
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Sex
MaleAge of model
12 weeksDuration of Study
1 monthDiet
Standard chowCage enrichment and housing
StandardTypes of outcomes measured
NeuroscoreStructural measure (i.e. IHC, EM, structural MRI)Vascular function (i.e. BBB integrity, CBF)Details of Outcomes Measured
Lesion volume, neuroscore, MRI (T", FA and ADC)
Standard Operating Procedures for Intervention
-Strengths of Model
- Focal
- Welfare and survival improvedReference to published procedure
DOI: 10.1242/dmm.029108
Image
Ischaemic stroke following occlusion (C57BL/6J) model
Ischemic stroke