- Overview
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Species
RatStrain common name
-Full nomenclature
-Genetic background
Sprague DawleyType of model
Ischemic stroke - Availability
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Principal investigator
Claire GibsonInstitution
University of NottinghamNamed contact
Claire GibsonContact email
claire.gibson@nottingham.ac.ukSource of model
SupplierSupplier code
Charles RiverMTA Holder and Issuing Organisation:
- - Intervention details
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Intervention
OcclusionAge at Surgery
11-12 weeksAnaesthetic
Isoflurane (induction 5%; maintenance 2% in N2O/O2, 30%/70%)Analgesics
Immediately prior to MCAO, all rats received subcutaneous Buprenorphine (0.003mg/kg, Vetergesic) along with Marcaine (2mg/kg, 0.25% w/v, AstraZeneca, UK) applied directly to the skin surrounding the wound site at the point of wound suture. All rats receivAntibiotics
NoDuration of occlusion is temporary
Method for inducing the occlusion is mechanical
Mechanical Occlusion Type is filament (doccol filament)
- Study details
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Sex
MaleAge of model
11 weeksDuration of Study
1 monthDiet
Standard chowCage enrichment and housing
Group housing, double deckerTypes of outcomes measured
Neurobehaviour (i.e. sensory, motor, cognition)Vascular function (i.e. BBB integrity, CBF)Details of Outcomes Measured
MRI (T2, ADC and FA), Behaviour (staircase test to assess forelimb function, adhesive test to assess sensorimotor function, cylinder test to assess forelimb asymmetry)
Strengths of Model
- Reproducible
- Good survival and wellbeing measuresReference to published procedure
DOI: 10.1371/journal.pone.0209370
Image
Ischaemic stroke following occlusion (Sprague Dawley) model
Ischemic stroke