Hypoperfusion to the brain

Overview: Species

Mouse

Strain common name

-

Full nomenclature

-

Genetic background

C57BL/6J

Type of model

Vascular cognitive impairment (i.e. chronic hypoperfusion)
Availability: Principal investigator

Tracy Farr

Institution

University of Nottingham

Named contact

Tracy Farr

Contact email

tracy.farr@nottingham.ac.uk

Source of model

Supplier

Supplier code

Charles River
Intervention details: Intervention

Stenosis

Age at Surgery

12-14 weeks

Anaesthetic

Isoflurane

Analgesics

Paracetamol in drinking water. Lidocaine presurgery. EMLA around incision site.

Antibiotics

None

Additional comments about stenosis: coils placed 24h apart
Study details: Sex

Male

Age of model

12-14 weeks

Duration of Study

Up to 6 months

Diet

Standard chow

Cage enrichment and housing

Group housed with standard conditions

Types of outcomes measured

Neurobehaviour (i.e. sensory, motor, cognition)

Neurophysiology (i.e. fMRI, EEG)

Omics (i.e transcriptomics, proteomics, metabolomics)

Structural measure (i.e. IHC, EM, structural MRI)

Details of Outcomes Measured

Behaviour (RAM, water maze, Y maze, NOR), structural MRI, fMRI, connectomics

Strengths of Model

- Straightforward.
- Involves gradual stenosis which is most replicable to that seen in human condition.

Limitations of Model

- Behaviour deficits have been unreliable and hard to replicate.