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Nature communications
Published

Predicting cell type-specific epigenomic profiles accounting for distal genetic effects

Authors

Alan E Murphy, William Beardall, Marek Rei, Mike Phuycharoen, Nathan G Skene

Abstract

Nat Commun. 2024 Nov 16;15(1):9951. doi: 10.1038/s41467-024-54441-5.

ABSTRACT

Understanding how genetic variants affect the epigenome is key to interpreting GWAS, yet profiling these effects across the non-coding genome remains challenging due to experimental scalability. This necessitates accurate computational models. Existing machine learning approaches, while progressively improving, are confined to the cell types they were trained on, limiting their applicability. Here, we introduce Enformer Celltyping, a deep learning model which incorporates distal effects of DNA interactions, up to 100,000 base-pairs away, to predict epigenetic signals in previously unseen cell types. Using DNA and chromatin accessibility data for epigenetic imputation, Enformer Celltyping outperforms current best-in-class approaches and generalises across cell types and biological regions. Moreover, we propose a framework for evaluating models on genetic variant effect prediction using regulatory quantitative trait loci mapping studies, highlighting current limitations in genomic deep learning models. Despite this, Enformer Celltyping can also be used to study cell type-specific genetic enrichment of complex traits.

PMID:39550354 | DOI:10.1038/s41467-024-54441-5